mouse anti hsp60 antibody Search Results


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Biomol GmbH monoclonal hsp60 (mouse) antibody (anti-hsp60) (1∶4000, overnight, 4°c)
A Mitochondrial proteins in WT and mutants grisea and PaCox17 ::ble were analysed with antibodies against <t>HSP60</t> after transfer to a PVDF membrane. HSP60 levels are increased in the two mutants. B Protein levels of LON protease (PaLON) are moderately increased in the mutants compared to the WT. Below each immunodetection a densitometric analysis of signal intensities (x-fold level compared to the WT) is shown. Intensities of the PaPORIN signals were used for normalisation. UniProt accession numbers: PaLON: B2AZ54; PaHSP60: B2B270 and PaPORIN: B2B736.
Monoclonal Hsp60 (Mouse) Antibody (Anti Hsp60) (1∶4000, Overnight, 4°C), supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A Mitochondrial proteins in WT and mutants grisea and PaCox17 ::ble were analysed with antibodies against HSP60 after transfer to a PVDF membrane. HSP60 levels are increased in the two mutants. B Protein levels of LON protease (PaLON) are moderately increased in the mutants compared to the WT. Below each immunodetection a densitometric analysis of signal intensities (x-fold level compared to the WT) is shown. Intensities of the PaPORIN signals were used for normalisation. UniProt accession numbers: PaLON: B2AZ54; PaHSP60: B2B270 and PaPORIN: B2B736.

Journal: PLoS ONE

Article Title: Alternative Oxidase Dependent Respiration Leads to an Increased Mitochondrial Content in Two Long-Lived Mutants of the Ageing Model Podospora anserina

doi: 10.1371/journal.pone.0016620

Figure Lengend Snippet: A Mitochondrial proteins in WT and mutants grisea and PaCox17 ::ble were analysed with antibodies against HSP60 after transfer to a PVDF membrane. HSP60 levels are increased in the two mutants. B Protein levels of LON protease (PaLON) are moderately increased in the mutants compared to the WT. Below each immunodetection a densitometric analysis of signal intensities (x-fold level compared to the WT) is shown. Intensities of the PaPORIN signals were used for normalisation. UniProt accession numbers: PaLON: B2AZ54; PaHSP60: B2B270 and PaPORIN: B2B736.

Article Snippet: Membranes were incubated in blocking buffer (Li-Cor, Lincoln, NE, USA) for 1 h at RT and subsequently probed with a polyclonal P. anserina PaLON protease antibody (Anti-PaLON) (1∶1500, overnight, 4°C), polyclonal P. anserina PaPRX1 (mitochondrial peroxiredoxin) antibody (Anti-PaPRX1) (1∶2000, overnight, 4°C), polyclonal P. anserina PaIAP (i-AAA protease) antibody (Anti-PaIAP) (1∶5000, overnight, 4°C), polyclonal P. anserina PaCLPP (CLP protease subunit) antibody (Anti-PaCLPP) (1∶400, overnight, 4°C) and a monoclonal HSP60 (mouse) antibody (Anti-HSP60) (1∶4000, overnight, 4°C) from Biomol Stressgen, Hamburg, Germany.

Techniques: Immunodetection